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Related Experiment Videos

Variability in commercial histoplasma complement fixation antigens.

D S Leland1, S E Zimmerman, E B Cunningham

  • 1Indiana University Medical Center, Indianapolis 46202-5200.

Journal of Clinical Microbiology
|August 1, 1991
PubMed
Summary
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Immuno-Mycologics (IMMY) antigens showed higher reactivity for detecting histoplasma antibodies compared to Meridian Diagnostics antigens. IMMY antigens identified more positive cases, especially in confirmed histoplasmosis patients.

Area of Science:

  • Medical Microbiology
  • Immunology
  • Infectious Diseases

Background:

  • Histoplasmosis diagnosis relies on serological tests.
  • Antigen performance can vary between manufacturers.
  • Accurate detection of histoplasma antibodies is crucial for timely treatment.

Purpose of the Study:

  • To compare the reactivity of Immuno-Mycologics (IMMY) and Meridian Diagnostics histoplasmal antibody complement fixation test antigens.
  • To evaluate the diagnostic performance of IMMY antigens against Meridian antigens.

Main Methods:

  • Retesting 1,386 samples using IMMY histoplasmal yeast (HY) and mycelial (HM) antibody complement fixation test antigens.
  • Comparing IMMY results with initial results obtained using Meridian Diagnostics antigens.
  • Analyzing discrepancies in antibody titers and identifying false negatives.

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Main Results:

  • IMMY detected histoplasma antibody (≥1:16) in 20% of HY and 5% of HM samples with low Meridian titers (<1:8).
  • IMMY titers were ≥4-fold higher than Meridian titers in 39% of HY and 54% of HM samples with high initial titers (≥1:8).
  • 52% of confirmed histoplasmosis cases were negative with Meridian antigens but positive with IMMY antigens.

Conclusions:

  • Meridian Diagnostics antigens demonstrated lower reactivity with human histoplasmal antibody.
  • IMMY antigens offer improved sensitivity for histoplasma antibody detection.
  • Re-evaluation of diagnostic assays is important for accurate histoplasmosis diagnosis.