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Related Experiment Videos

Ig allotypic inclusion does not prevent B cell development or response.

Maria-Gabriela Velez1, Melissa Kane, Sucai Liu

  • 1Integrated Department of Immunology, National Jewish Medical and Research Center, University of Colorado Health Sciences Center, Denver, CO 80206, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|July 10, 2007
PubMed
Summary
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B cells expressing two different immunoglobulin kappa light chains (allotype included) are present in mature B cell populations. These dual kappa B cells show functional responses in vitro and in vivo, suggesting roles in immune responses.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • B cells typically express only one type of immunoglobulin (Ig) light chain.
  • Rare instances of B cells expressing two distinct Ig kappa light chains (allotype included) have been observed.
  • The frequency and functional significance of these dual kappa B cells remain largely uncharacterized.

Purpose of the Study:

  • To determine the frequency and functional capacity of B cells expressing two distinct Ig kappa light chains.
  • To investigate the in vitro and in vivo responsiveness of these dual kappa B cells.
  • To explore the potential role of allotype-included B cells in physiological and pathological immune responses.

Main Methods:

  • Gene-targeted mice carrying human and mouse Ig kappa constant region genes were analyzed.

Related Experiment Videos

  • Flow cytometry and various stimulation assays were employed to assess B cell populations.
  • In vitro activation assays and in vivo immune response models were utilized.

    • Main Results:

    • Dual kappa B cells constitute 1.4-3% of all B cells across follicular, marginal zone, and B1 subsets.
    • These cells exhibit proliferation and activation marker up-regulation upon anti-IgM stimulation, similar to single kappa B cells.
    • Suboptimal in vitro responses were observed when activation was mediated by reagents binding only one kappa chain, suggesting reduced B cell receptor cross-linking.

    Conclusions:

    • Allotype-included B cells are a normal component of the mature mouse B cell repertoire.
    • Dual kappa B cells are responsive to B cell receptor stimulation both in vitro and in vivo.
    • The coexpression of autoreactive and nonautoreactive B cell receptors on these cells suggests a potential role in diverse immune responses, including autoimmunity.