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Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy

Tassula Proikas-Cezanne1, Sabine Ruckerbauer, York-Dieter Stierhof

  • 1Autophagy Laboratory, Department of Molecular Biology, University of Tuebingen, Auf der Morgenstelle 15, 72076 Tuebingen, Germany. tassula.proikas-cezanne@uni-tuebingen.de

FEBS Letters
|July 10, 2007
PubMed

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Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...