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In vitro antimicrobial activity of peroxide-based bleaching agents
Marcelo Henrique Napimoga1, Rogério de Oliveira, André Figueiredo Reis
1Laboratory of Molecular Biology, University of Uberaba, MG, Brazil.
Quintessence International (Berlin, Germany : 1985)
|July 13, 2007
Summary
Four commercial bleaching agents demonstrated antibacterial activity against oral pathogens. Peroxide-based agents showed effectiveness comparable to or exceeding chlorhexidine, with some microorganisms exhibiting resistance.
Area of Science:
- Dental Materials Science
- Microbiology
- Oral Health
Background:
- Dental bleaching agents are widely used for aesthetic purposes.
- Concerns exist regarding the potential antimicrobial effects of these agents on oral flora.
- Understanding the antibacterial properties of bleaching agents is crucial for patient safety and oral health.
Purpose of the Study:
- To evaluate the antibacterial activity of four commercial dental bleaching agents.
- To compare the efficacy of bleaching agents against common oral pathogens and Staphylococcus aureus.
- To determine if antimicrobial activity is linked to the peroxide content of the bleaching agents.
Main Methods:
- Agar diffusion assay was employed to assess inhibition zones.
- Four commercial bleaching agents (Day White, Colgate Platinum, Whiteness 10%, 16%) were tested.
- Chlorhexidine (positive control) and distilled water (negative control) were used for comparison.
- Six oral pathogens (Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Candida albicans, Lactobacillus casei, Lactobacillus acidophilus) and Staphylococcus aureus were evaluated.
- Statistical analysis using 2-way ANOVA and Tukey test was performed.
Main Results:
- All tested bleaching agents and chlorhexidine exhibited antibacterial inhibition zones.
- Antimicrobial activity was found to be dependent on peroxide-based bleaching agents.
- Most bleaching agents produced inhibition zones similar to or larger than chlorhexidine.
- Candida albicans, Lactobacillus casei, and Lactobacillus acidophilus demonstrated the highest resistance to the agents.
Conclusions:
- Commercial bleaching agents possess significant antibacterial properties against a range of oral microorganisms.
- Peroxide concentration appears to be a key factor in the antimicrobial efficacy of these agents.
- While generally effective, certain microorganisms like Candida albicans and Lactobacillus species show reduced susceptibility.
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