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Which test chambers should be used for acetone, ethanol, and water solutions when patch testing?

Marléne Isaksson1, Birgitta Gruvberger, Malin Frick-Engfeldt

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Area of Science:

  • Dermatology
  • Allergen Testing
  • Patch Testing

Background:

  • Standard volumes for patch testing chambers (Finn and van der Bend) are generally accepted but their adequacy is questioned.
  • Optimizing patch test volumes is crucial for accurate diagnostic results in dermatology.

Purpose of the Study:

  • To evaluate the adequacy of recommended volumes for Finn and van der Bend chambers.
  • To compare the efficacy of different chamber types for testing water, ethanol, and acetone solutions.

Main Methods:

  • 12 volunteers underwent patch testing on the back using green-coloured water, ethanol, and acetone solutions.
  • Solutions were applied in both Finn and van der Bend chambers on two separate occasions.
  • Test sites were assessed on Day 2, with scoring based on coverage and spreading of the test solution.

Main Results:

  • Ethanol solutions resulted in 100% positive reactions in both chamber types, with significant spreading in 23/24 Finn Chambers.
  • No spreading was observed in van der Bend chambers for any of the tested solutions (water, ethanol, acetone).
  • Water and acetone solutions showed 0-8% negative reactions in both Finn and van der Bend chambers.

Conclusions:

  • Water solutions are best tested using Finn Chambers.
  • Ethanol and acetone solutions demonstrate superior performance and reduced spreading when tested in van der Bend chambers.
  • The study suggests specific chamber recommendations based on the type of solution tested for improved patch testing accuracy.