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Updated: Jul 13, 2026
11:34
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Published on: August 9, 2019
SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands
Regina Stoltenburg1, Christine Reinemann, Beate Strehlitz
1UFZ, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
Biomolecular Engineering
|July 14, 2007
Summary
Systematic evolution of ligands by exponential enrichment (SELEX) is a method to develop aptamers. These DNA or RNA molecules offer high affinity and specificity for diverse targets, finding applications in diagnostics and therapeutics.
Area of Science:
- Biotechnology
- Molecular Biology
- Biochemistry
Background:
- Aptamers are high-affinity oligonucleotide ligands selected through SELEX.
- They recognize diverse targets, including proteins, small molecules, and metal ions.
- Aptamers have broad applications in research, drug development, diagnostics, and therapeutics.
Purpose of the Study:
- To review the SELEX method for aptamer development.
- To provide an overview of aptamer technologies, advantages, limitations, and applications.
Main Methods:
- SELEX (Systematic Evolution of Ligands by Exponential Enrichment) is the primary method discussed.
- Modifications to SELEX have improved efficiency, speed, affinity, and automation.
Main Results:
- SELEX enables the selection of high-affinity aptamers for various targets.
- Numerous modifications have enhanced SELEX efficiency and applicability.
- Aptamers are versatile tools in molecular recognition and binding applications.
Conclusions:
- SELEX is a powerful technology for generating aptamers with high specificity and affinity.
- Aptamers are valuable tools with significant potential in diagnostics, therapeutics, and analytical applications.
- Ongoing SELEX advancements continue to expand aptamer utility and accessibility.
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