Related Experiment Video
Updated: Jul 13, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Regulation of respiratory genes by ResD-ResE signal transduction system in Bacillus subtilis
Hao Geng1, Peter Zuber, Michiko M Nakano
1Department of Environmental System, Oregon Health and Science University, Beaverton, Oregon, USA.
Abstract:
Successful respiration in Bacillus subtilis using oxygen or nitrate as the terminal electron acceptor requires the ResD-ResE signal transduction system. Although transcription of ResDE-controlled genes is induced at the stationary phase of aerobic growth, it is induced to a higher extent upon oxygen limitation. Furthermore, maximal transcriptional activation requires not only oxygen limitation, but also nitric oxide (NO). Oxygen limitation likely results in conversion of the ResE sensor kinase activity from a phosphatase-dominant to a kinase-dominant mode. In addition, low oxygen levels promote the production and maintenance of NO during nitrate respiration, which leads to elimination of the repression exerted by the NO-sensitive transcriptional regulator NsrR. ResD, after undergoing ResE-mediated phosphorylation, interacts with the C-terminal domain of the alpha subunit of RNA polymerase to activate transcription initiation at ResDE-controlled promoters.
More Related Videos
10:18A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
Published on: April 25, 2015
12:24DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Related Concept Videos
Global Regulatory Systems
Gene Regulation in Microbial Communities: Quorum Sensing
Gene Regulation During Sporulation
Regulation of Bacterial Virulence
Transcriptional Regulation: Riboswitches
Other Stress Responses in Bacteria