[Reconstruction of ossicular chain by in situ bone tissue engineering technique]

To make ossicular prosthesis using little columnar acellular cancellous bone combined with bone morphogenetic protein 2 (BMP-2) and implant into rabbits' acoustic vesicle to observe the osteogenesis in situ.

To prepare the acellular cancellous bone combined with BMP-2 as ossicular prosthesis, the same material without BMP-2 were used in the control group. By retroauricular approach, a hole was made in the posterolateral bone wall of the acoustic vesicle and the prepared materials was implanted. After 3 month, the osteogenesis of the prosthesis with macroscopical anatomy and pathology were observed.

The rabbits recovered soon after surgery, the implanted acellular cancellous bones were banded tightly with the bone of the acoustic vesicle bone wall. The surface of all materials was covered with mucosa while osteogenesis was found in the material with BMP-2.

The acellular cancellous bone with BMP-2 could induce osteogenesis in acoustic vesicle, so this material might be use to reconstruct the defects of ossicular chain.