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Updated: Jul 13, 2026

Remote Laboratory Management: Respiratory Virus Diagnostics
Published on: April 6, 2019
Sterilization of a biological safety cabinet by vaporized KMT reagent
Nori Kida1, Yasushi Mochizuki, Fumiaki Taguchi
1Niigata Quarantine Station, Ministry of Health, Labour and Welfare, 1-5-4, Ryugashima, Chuo-ku, Niigata, Niigata 950-0072, Japan. kida-nori@keneki.go.jp
Abstract:
To investigate effective methods to sterilize a biological safety cabinet contaminated with Bacillus spores, a sterilization study was performed using the vaporized KMT reagent. Five kinds of test carriers inoculated with Bacillus subtilis spores, which had been prepared in-house, were used as indicators. The KMT reagent was placed in the middle of the work-area (1.2 m-wide, Class II type A cabinet). Approximately 300 ml of vaporized KMT reagent could bring about complete sterilization of this cabinet by 24 h of exposure without severe corrosion. Vaporized KMT reagent could be used for the safe and easy sterilization of cabinets contaminated with Bacillus spores.
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