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A biochemical assay for acetylcholinesterase activity in PC12 cells
Paul J Schwartz1, Jay A Blundon, Elizabeth M Adler
1Department of Neurological Surgery, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA.
Abstract:
This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.
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