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Related Experiment Video

Updated: Jul 13, 2026

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium
09:45

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[Slow Ca2'-binding Mg2+- EDTA buffer for intracellular perfusion].

G N Berestovskiĭ, A A Kataev, A A Grinevich

    Biofizika
    |July 19, 2007
    PubMed
    Summary

    A novel buffer system using EDTA and Mg2+ can control calcium ion (Ca2+) binding kinetics in cell studies. This buffer offers slower Ca2+ binding than the cytoplasm, aiding research on calcium channels.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Biochemistry

    Context:

    • Studies involving perfused cells and membrane fragments require precise control over calcium ion (Ca2+) concentrations.
    • Potential-dependent Ca2+ channels and Ca2+-activated channels are crucial for cellular signaling and function.

    Purpose:

    • To evaluate the utility of a buffer system with ethylenediaminetetraacetic acid (EDTA) in excess of magnesium ions (Mg2+) for controlling Ca2+ binding kinetics.
    • To calculate and analyze the kinetic characteristics of this proposed buffer system.

    Summary:

    • A buffer with excess EDTA over Mg2+ was proposed and its kinetic characteristics were calculated.
    • This buffer can achieve Ca2+ binding with a characteristic time of up to tens of milliseconds, depending on component proportions.

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    Last Updated: Jul 13, 2026

    Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium
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  • This binding time is significantly slower than the typical cytoplasmic Ca2+ binding time (≤1 ms), even with large calcium signals.

    • Impact:

    • Provides a tool for researchers studying calcium channel function and cellular calcium dynamics.
    • Enables more controlled experimental conditions in electrophysiology and cell signaling research.
    • Facilitates a deeper understanding of calcium buffering mechanisms in biological systems.