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High Throughput Co-culture Assays for the Investigation of Microbial Interactions
Published on: October 15, 2019
Isolation, structure, and coccidiostat activity of coccidiostatin A
Hiranthi Jayasuriya1, Ziqiang Guan, Anne W Dombrowski
1Merck Research Laboratories, P. O. Box 2000, Rahway, New Jersey 07065, USA. hiranthi_jayasuriya@merck.com
Abstract:
Coccidiosis is one of the more common and costly diseases in poultry that is caused by various Eimeria species. In our quest to discover coccidiostats from natural products, we discovered a microbial fermentation extract that exhibited in vivo anticoccidial activity. Fractionation of this extract led to the discovery of two potent antiprotozoals, emecorrugatin A (1) and coccidiostatin A (2). The former compound exhibited only in vitro activity, whereas the latter new compound exhibited in vivo activity against Eimeria species in chickens at 150 ppm dosed in chicken feed. The isolation, structure elucidation, relative configuration, and activity of coccidiostatin A (2) are described.
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