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Updated: Jul 13, 2026
08:40
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
Published on: October 21, 2022
What's taking so long? S-phase entry from quiescence versus proliferation
1Department of Molecular Biology, Lewis Thomas Laboratory, Room 140, Princeton University, Princeton, New Jersey 08544-1014, USA. hcoller@princeton.edu
Nature Reviews. Molecular Cell Biology
|July 20, 2007
Summary
Mammalian cells temporarily exit the cell cycle in response to cues. Upon re-entry, these cells experience a prolonged G1 phase before DNA replication, a delay that remains poorly understood.
Area of Science:
- Cell biology
- Mammalian cell cycle regulation
Background:
- Cells can temporarily withdraw from the cell cycle in response to extracellular signals.
- Re-entering cells exhibit a significantly extended G1 phase compared to continuously cycling cells.
Purpose of the Study:
- To investigate the underlying mechanisms causing the prolonged G1 phase in cells re-entering the cell cycle.
- To elucidate the molecular events responsible for the extended G1 delay.
Main Methods:
- The study likely involves cell culture techniques to induce cell cycle arrest and re-entry.
- Analysis of cell cycle progression using methods like flow cytometry.
- Potential investigation of specific gene or protein expression changes during G1 phase.
Main Results:
- The research aims to identify the specific molecular factors or pathways responsible for the extended G1 duration.
- Quantification of the extra time required in G1 phase for quiescent cells.
Conclusions:
- Understanding the G1 delay is crucial for comprehending cell cycle control and cellular responses to environmental cues.
- Further research is needed to fully resolve the molecular basis of this extended G1 phase.
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00:54Interphase
The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
00:56Interphase
The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
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Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
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02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...