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Morphogenesis and growth kinetics of Fusarium verticillioides

Carlos Alberto Granjo1, Tatiana Alves dos Reis, Walderez Gambale

  • 1Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil.

Mycopathologia
|July 20, 2007
PubMed

Insights

This study analyzed the growth and development of Fusarium verticillioides. A fluorescence method differentiated viable and non-viable fungal cells, revealing asynchronous morphogenesis.

Area of Science:

  • Mycology
  • Plant Pathology
  • Microbial Physiology

Background:

  • Fusarium verticillioides is a toxigenic fungus impacting agriculture.
  • Understanding its growth and development is crucial for disease management.
  • Existing methods for assessing fungal viability and morphology have limitations.

Purpose of the Study:

  • To analyze the growth kinetics of toxigenic Fusarium verticillioides strains.
  • To investigate the morphogenesis of F. verticillioides during different developmental stages.
  • To evaluate a fluorescence-based method for differentiating viable and non-viable fungal cells.

Main Methods:

  • Growth curves were generated using mycelial dry weight over 60 days.
  • Morphogenesis was studied using Fluorescein Diacetate (FDA) and Ethidium Bromide (EB) fluorescence.
  • Viable cells fluoresce green, while non-viable cells exhibit red fluorescence.

Main Results:

  • Growth curves followed a homogeneous pattern, similar to ideal growth.
  • Morphogenesis was asynchronous, with diverse fungal forms observed up to 50 days.
  • The FDA/EB fluorescence method effectively distinguished viable from non-viable F. verticillioides cells.

Conclusions:

  • Fusarium verticillioides exhibits asynchronous morphogenesis.
  • The fluorescence method is a valuable tool for assessing fungal viability and developmental stages.
  • This research provides insights into the life cycle of a significant plant pathogen.

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