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[Study on the localization of a major allergen Der f 2 by fluorescence immunohistochemical method]
Meng Li1, Ying Bao, Zhi-Gang Liu
1Allergy and Immunology Institute. Shenzhen University, Shenzhen 518060, China.
Objective:
To localize Der f 2 in the body of Dermatophagoides farinae.
Methods:
Live mites were embedded. Serial mite paraffin sections were made and checked under light microscope. The sections were incubated with anti-recombinant Der f 2 monoclonal antibody as the first antibody. They were then reacted with the fluorescent isothiocyanate conjugated goat anti-mouse IgG as the secondary antibody. The sections were examined with fluorescent light microscopy.
Results:
Der f 2 reacting with immunofluorescent antibodies was found localized in the tissue and contents of the mid-gut of the mite.
Conclusion:
The major allergen Der f 2 distributes in the gut and fecal pellets of Dermatophagoides farinae.
