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Updated: Jul 13, 2026
Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
Published on: February 28, 2019
[A preliminary study on preservation methods of Blastocystis hominis]
Chun-Lin Tian1, Xiao-Ling Wan, Deng-Yu Liu
1Department of Parasitology, Guangxi Medical University, Nanning 530021, China.
Objective:
To observe the effect of different cryoprotective agents and temperature factors on the viability of Blastocystis hominis so as to explore the ideal method for preservation of B. hominis.
Methods:
B. hominis agents were obtained from a patient's fecal specimen. Having washed by normal saline and divided into tubes, the samples were cryopreserved in -20 degrees C refrigerator or in -196 degrees C liquid nitrogen with 10% DMSO, 40% glycerol and 15% ethylene glycol respectively. The thawed B. hominis agents were then used for culture. By trypan blue staining and microscopy, the viability and proliferation of those resuscitative cells were investigated.
Results:
B. hominis survived for 3 weeks at 18 degrees C-20 degrees C while less than 1 week at 4 degrees C-6 degrees C. When stored in -20 degrees C refrigerator or liquid nitrogen with cryoprotective agents, they survived for more than 3 months. The cryopreservation with 40% glycerol at -196 degrees C for 6 months resulted in 41.7% viability of the revivified cells. Cleavage cells were easily observed after culturing for 72 hours.
Conclusion:
Preserving B. hominis in liquid nitrogen with 40% glycerol is an optimal cryopreservation protocol.
