[Evaluation of toxification in acid and alkalescent industry liquid wastes from paper mill using C. elegans]

Model organism C. elegans was introduced into the toxicity identification evaluation (TIE) procedure to determine the toxification of acid and alkalescent industry liquid wastes from paper mill via analyzing the life span, days corresponding to 50% lethal, reproductive rate, reproductive capacity and locomotion behaviors in worms. Our data indicate that the toxic influence order of different samples on life span was: 2nd > 4th > 1st > 3rd, the toxic influence order of different samples on generation time was: 2nd > 1st > 3rd approximately 4th, the toxic influence order of different samples on brood size and head thrashes was similar: 1st approximately 2nd > 3rd approximately 4th, the toxic influence order of different samples on body bends was: 1st approximately 2nd > 3rd > 4th, and the 2nd sample always had more significantly toxic effects on different phenotypes. Thus, the toxicity of acid industry liquid waste from the paper mill relatively had the most severe multiple effects, whereas the toxicity of alkalescent industry liquid and grain-like wastes only exhibited limited effects.