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Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes
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Iron absorption by Belgrade rat pups during lactation.

Khristy Thompson1, Ramon M Molina, Thomas Donaghey

  • 1Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA.

American Journal of Physiology. Gastrointestinal and Liver Physiology
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Divalent metal transporter-1 (DMT1) is not essential for iron assimilation from milk in neonatal rats. Defective DMT1 function in Belgrade rats impacts iron absorption but not early milk-based iron uptake.

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Area of Science:

  • Physiology
  • Biochemistry
  • Genetics

Background:

  • Divalent metal transporter-1 (DMT1) is crucial for dietary nonheme iron absorption.
  • Belgrade (b) rats possess a mutation in the DMT1 gene, leading to defective iron metabolism.

Purpose of the Study:

  • To investigate the role of DMT1 in neonatal iron assimilation in rats.
  • To compare iron uptake and distribution in rats with and without functional DMT1 during early development.

Main Methods:

  • Cross-fostering of Belgrade (b/b) and heterozygous (b/+) rat pups to dams injected with radioisotope-labeled iron ((59)FeCl(3)).
  • Measurement of (59)Fe distribution in tissues at weaning (day 21).
  • Pharmacokinetic study of (59)FeCl(3) absorption via intragastric gavage in 21-day-old rats.

Main Results:

  • b/b pups exhibited lower blood (59)Fe levels but higher tissue (59)Fe levels compared to b/+ controls at weaning.
  • Post-gavage, b/b rats showed significantly lower blood and tissue (59)Fe levels, indicating impaired intestinal iron absorption.
  • These findings suggest DMT1's role in intestinal iron absorption is more prominent after weaning.

Conclusions:

  • DMT1 function is not essential for iron assimilation from milk during early rat development.
  • Impaired DMT1 function in b/b rats affects post-weaning iron absorption but not milk-derived iron uptake.
  • Neonatal iron assimilation in rats can occur independently of functional DMT1.