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Updated: Jul 13, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
[The molecular characteristic of Staphylococcus aureus and Staphylococcus epidermidis strains isolated from clinical
Daria Zdzalik1, Aleksandra Dominiak, Hanna Gałkowska
1Zespół Patofizjologii i Immunologii Transplantacyjnej w Zakładzie Badawczo-Leczniczym Chirurgii Transplantacyjnej Instytutu Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN w Warszawie.
Abstract:
The aim of study was the molecular characteristic of S. aureus and S. epidermidis isolates obtained from skin surface, wounds, deep tissues of hospitalized patients and from skin surface of non-hospitalized patients. Genes encoding virulence factors were examined using PCR reaction and specific primers. Genes encoding adhesinsfnbA and cna and gene eta for epidermolytic toxin were mostly present in S. aureus isolates coming from wounds and deep tissues compared to these from skin surface. Gene atlE encoding autolysin of S. epidermidis was detected in all studied isolates, whereas gene icaAB was present in almost all isolates. Comparison of results obtained by PCR and conventional method of the resistance to methicillin estimation showed discrepances suggesting the need for using of both methods in some clinically difficult cases of S. aureus infection.
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