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Rotavirus infection among infants and young children in Chennai, South India

P Saravanan1, S Ananthan, M Ananthasubramanian

  • 1Department of Microbiology, Dr. ALM PGIBMS, University of Madras, Taramani Campus, Chennai - 600 113, India.

Indian Journal of Medical Microbiology
|July 24, 2007
PubMed

Insights

Group A rotavirus causes severe childhood gastroenteritis globally. This study identified predominant G2 P[4,8] strains in South India, highlighting the need for molecular characterization for vaccine development.

Area of Science:

  • Virology
  • Epidemiology
  • Public Health

Background:

  • Group A rotavirus is a leading cause of severe gastroenteritis in children worldwide.
  • Epidemiological studies are crucial for vaccine development and monitoring emerging rotavirus strains.
  • Understanding rotavirus diversity is essential for global health initiatives.

Purpose of the Study:

  • To determine the prevalence and characterize Group A rotavirus strains in children with acute diarrhea in South India.
  • To analyze the antigenic and genomic diversity of circulating rotaviruses.
  • To provide data for rotavirus vaccine evaluation programs.

Main Methods:

  • Monoclonal antibody (MAb)-based ELISA and polyacrylamide gel electrophoresis (PAGE) were used for rotavirus characterization.
  • Stool samples were collected from 745 children aged 0-3 years with acute diarrhea.
  • Antigenic (group, subgroup, serotype) and genomic (VP7 and VP4 genotype) analyses were performed.

Main Results:

  • Rotavirus was detected in 168 out of 745 stool samples (22.6%).
  • The predominant strains identified were short electropherotype (E-type), subgroup I (SGI), and serotype G2, found in 66.1% of positive samples.
  • High genomic diversity was observed, with 10 different E-types and mixed genotypes including G2 P[4,8] and G1-G2 P[4,8].

Conclusions:

  • This study describes the predominant rotavirus strains circulating in South India.
  • The findings underscore the significant genomic diversity of rotaviruses in the region.
  • Further molecular characterization of rotaviruses is essential for effective vaccine strategies and evaluation.
Abstract

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