Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
Hand hygiene01:23

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
Surface Membrane Barriers01:18

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enterococcus Faecalis Biofilm. Formation and Development in Vitro Observed by Scanning Electron Microscopy.

Acta odontologica latinoamericana : AOL·2016
Same author

Antibacterial action of calcium hydroxide vehicles and calcium hydroxide pastes.

Journal of investigative and clinical dentistry·2012
Same author

Dye diffusion in instrumented root canals irrigated with different solutions.

Acta odontologica latinoamericana : AOL·2010
Same author

Protein content in irrigating solutions in contact with pulp tissue.

Acta odontologica latinoamericana : AOL·2008
Same author

Influence of filling procedures and the partial removal of filling on the seal of root canals filled with gutta-percha and glass ionomer cement.

Acta odontologica latinoamericana : AOL·2008
Same author

Detection of viable and viable nonculturable Vibrio cholerae O1 through cultures and immunofluorescence in the Tucumán rivers, Argentina.

Revista da Sociedade Brasileira de Medicina Tropical·2007

Related Experiment Video

Updated: Jul 13, 2026

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection
07:57

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

Published on: June 24, 2025

In vitro antibacterial effect of different irrigating solutions on Enterococcus faecalis.

María de los Angeles Bulacio1, Rosa Cangemi, Marta Cecilia

  • 1Department of Endodontics, Faculty of Dentistry, National University of Tucumán, Argentina. maritabulacio@hotmail.com

Acta Odontologica Latinoamericana : AOL
|July 25, 2007
PubMed
Summary

This study compared the effectiveness of sodium hypochlorite (NaOCl), chlorhexidine gluconate (CHX), and EDTA in eliminating Enterococcus faecalis. NaOCl demonstrated the strongest antibacterial effect on root dentin, completely inhibiting bacteria in the apical region for 24 hours.

More Related Videos

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
09:02

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

Published on: June 7, 2017

Related Experiment Videos

Last Updated: Jul 13, 2026

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection
07:57

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

Published on: June 24, 2025

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
09:02

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

Published on: June 7, 2017

Area of Science:

  • Endodontics
  • Microbiology
  • Biomaterials

Background:

  • Enterococcus faecalis is a common pathogen in persistent endodontic infections.
  • Effective intracanal disinfection is crucial for endodontic treatment success.
  • Evaluating irrigant efficacy against E. faecalis is essential for optimizing root canal therapy.

Purpose of the Study:

  • To compare the minimum inhibitory concentration (MIC) and antibacterial effect (AE) of 2.5% sodium hypochlorite (NaOCl), 0.2% chlorhexidine gluconate (CHX), and 17% ethylenediaminetetraacetic acid (EDTA) against Enterococcus faecalis.
  • To assess the efficacy of these irrigants on contaminated root dentin.

Main Methods:

  • MIC and agar diffusion assays were performed to determine the inhibitory concentrations and diffusion zones.
  • Antibacterial effect was evaluated on contaminated human root dentin (apical and middle portions).
  • Root dentin samples were incubated with irrigants at 37°C, and viable bacterial cells were counted at 0, 4, 8, and 24 hours.

Main Results:

  • MIC values were 0.2% for NaOCl and CHX, and below 5% for EDTA.
  • Agar diffusion showed inhibition zones of 21 mm for NaOCl, 14 mm for CHX, and 20 mm for EDTA.
  • NaOCl 2.5% completely inhibited E. faecalis for 24 hours in the apical area and 8 hours in the middle area.
  • CHX 0.2% reduced bacterial load by over 5 log CFU, while EDTA 17% reduced it by over 3 log CFU at all time points.

Conclusions:

  • 2.5% NaOCl exhibits superior antibacterial efficacy against Enterococcus faecalis in root dentin compared to 0.2% CHX and 17% EDTA.
  • NaOCl provides sustained antibacterial activity, particularly in the apical root canal region.
  • CHX and EDTA also demonstrate significant antibacterial effects, but to a lesser extent than NaOCl under the tested conditions.