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Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

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Published on: May 17, 2024

Protein content in irrigating solutions in contact with pulp tissue.

María Luisa de la Casa¹, María Mercedes Salas, María Elena López

¹Department of Endodoncy, School of Dentistry, Universidad Nacional de Tucumán, Argentina. mldelacasa@tucbbs.com.ar

Acta Odontologica Latinoamericana : AOL

|October 10, 2008

Summary

Sodium hypochlorite and calcium hydroxide effectively denature proteins in vital and necrotic pulp tissue. Other tested endodontic irrigants like chlorhexidine, tea, and distilled water showed no significant dissolving action.

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Area of Science:

Endodontics
Biochemistry
Dental Materials Science

Background:

Endodontic irrigating solutions are crucial for pulp tissue dissolution.
Understanding the protein-dissolving capacity of various irrigants is essential for effective root canal treatment.

Purpose of the Study:

To quantitatively and qualitatively evaluate the pulp tissue dissolving capacity of different endodontic irrigants in vitro.
To compare the effects of sodium hypochlorite, calcium hydroxide, chlorhexidine gluconate, tea, and distilled water on vital and necrotic pulp proteins.

Main Methods:

Vital and necrotic bovine pulp tissues were exposed to various irrigants (sodium hypochlorite, calcium hydroxide, chlorhexidine gluconate, tea, distilled water) at 37°C.
Total soluble pulp protein was measured using the Lowry method at 30 minutes, 90 minutes, and 20 hours.
Soluble protein bands were analyzed using 12% SDS-PAGE electrophoresis.

Main Results:

Both concentrations of sodium hypochlorite and calcium hydroxide demonstrated protein denaturation.
0.2% chlorhexidine gluconate, 1% tea, and distilled water exhibited no significant solvent action on pulp proteins.
Electrophoretic analysis confirmed protein denaturation by sodium hypochlorite and calcium hydroxide.

Conclusions:

Sodium hypochlorite and calcium hydroxide are effective in denaturing pulp tissue proteins.
Chlorhexidine gluconate, tea, and distilled water lack significant pulp tissue solvent action.
These findings highlight the differential protein-dissolving capabilities of common endodontic irrigants.