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Related Experiment Video

Updated: Jul 13, 2026

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
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Stabilizer-induced viscosity alteration biases nanoparticle sizing via dynamic light scattering.

Christian Fillafer1, Michael Wirth, Franz Gabor

  • 1Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, Vienna, Austria.

Langmuir : the ACS Journal of Surfaces and Colloids
|July 25, 2007
PubMed
Summary

Surfactant concentration significantly impacts nanoparticle size measurements using dynamic light scattering (DLS). Even small Pluronic F-68 changes (0.005-2%) can alter particle diameter by up to 65 nm due to viscosity shifts.

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Area of Science:

  • Colloid and surface science
  • Nanotechnology
  • Materials science

Background:

  • Accurate nanoparticle sizing is crucial for understanding size-dependent properties.
  • Dynamic light scattering (DLS) is a primary technique for particle size determination.
  • System complexity from surfactants and proteins often complicates DLS analysis.

Purpose of the Study:

  • To investigate the effect of Pluronic F-68 surfactant concentration on nanoparticle size determination by DLS.
  • To quantify the relationship between surfactant content and measured particle size.
  • To understand the underlying mechanisms causing size discrepancies.

Main Methods:

  • Dynamic light scattering (DLS) was used for particle size analysis.
  • A photometric assay monitored changes in surfactant concentration.

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Last Updated: Jul 13, 2026

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

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  • Correlations were established between surfactant levels and particle size variances.

    • Main Results:

    • Pluronic F-68 concentrations from 0.005% to 2% induced significant changes in measured particle size.
    • A 65 nm shift in diameter was observed for 200 nm particles with varying surfactant content.
    • These alterations are attributed to surfactant-induced viscosity fluctuations.

    Conclusions:

    • Surfactant concentration is a critical, often overlooked, factor in DLS measurements of nanoparticles.
    • Viscosity changes driven by surfactant levels directly impact particle size calculations via the Stokes-Einstein relation.
    • Accurate nanoparticle characterization requires careful control and consideration of surfactant concentrations.