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High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary
G Seguí-Lines1, J M A Gavina, J C D'Amaral
1Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4M1.
Abstract:
Dynamic ligand exchange-affinity capillary electrophoresis (DLE-ACE) is introduced as a convenient platform for assessing the conformational stability and relative affinity of a holoprotein to different ligands without off-line sample pretreatment, since ligand exchange and protein unfolding processes are integrated in-capillary during electromigration.
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