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Aminopeptidase activities present in tissues of Helix aspersa
M P Ennis1, G B Irvine, C H Williams
1Division of Biochemistry, School of Biology and Biochemistry, Queen's University, Belfast, UK.
Abstract:
1. Tissues of Helix aspersa have been examined for the presence of aminopeptidase activities. 2. Enzyme activities were detected using synthetic peptide substrates containing the fluorescent leaving group 7-amino-4-methyl coumarin. The methods used included continuous fluorimetric assay and activity staining of enzymes in non-denaturing polyacrylamide gels. 3. Several types of peptidase activity were detected, including three distinct aminopeptidases which differ in molecular size and substrate preference.