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Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state
1Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China.
Letters in Applied Microbiology
|July 27, 2007
Summary
This study optimized alpha-galactosidase production using Aspergillus foetidus in solid-state fermentation (SSF). The developed medium significantly increased enzyme activity, paving the way for industrial applications in food and feed industries.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Industrial Microbiology
Background:
- Alpha-galactosidase hydrolyzes raffinose oligosaccharides (RO), reducing flatulence from soybean products.
- Current methods for alpha-galactosidase production require optimization for industrial efficiency.
Purpose of the Study:
- To develop an optimal culture medium for alpha-galactosidase production by Aspergillus foetidus using solid-state fermentation (SSF).
- To enhance enzyme yield for industrial applications in the food and feed sectors.
Main Methods:
- Response Surface Methodology (RSM) and Central Composite Design (CCD) were employed to optimize fermentation medium composition.
- Fractional Factorial Design (FFD) was used to identify key factors influencing enzyme production.
- Key components optimized included wheat bran, soybean meal, KH(2)PO(4), MnSO(4).H(2)O, and CuSO(4).5H(2)O.
Main Results:
- The optimal fermentation medium comprised 8.2137 g wheat bran, 1.7843 g soybean meal, 0.001 g MnSO(4).H(2)O, and 0.001 g CuSO(4).5H(2)O per 10 g dry matter.
- Predicted alpha-galactosidase activity reached 2210.76 U g(-1) dry matter after 96 h incubation.
- Actual achieved activity was 2207.19 U g(-1) dry matter, validating the optimized medium.
Conclusions:
- Optimization of the solid substrate is crucial for increasing alpha-galactosidase activity.
- The developed SSF process shows potential for scale-up and industrial production of alpha-galactosidase.
- This research contributes to improving the utilization of soybean-derived products by reducing associated digestive discomfort.
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