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Updated: Jul 13, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Multifunctional envelope-type nano device for non-viral gene delivery: concept and application of Programmed
Kentaro Kogure1, Hidetaka Akita, Hideyoshi Harashima
1Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo City, Hokkaido 060-0812, Japan.
Abstract:
In this review, a new concept called "Programmed Packaging" is proposed for developing non-viral gene delivery systems. A Multifunctional Envelope-type Nano Device (MEND) was developed based on this concept, which can enter into cells via macropinocytosis and facilitate transfection activities as efficiently as an adenovirus. Intracellular trafficking of MEND was optimized in order to prevent lysosomal degradation and to enhance nuclear translocation. A quantitative analytical method (CIDIQ) was also established to evaluate each step in intracellular trafficking of genes as well as carriers. A comparative study between Lipofectamin and an adenovirus showed that intra-nuclear disposition, which includes the transcriptional process, translational process, etc., can be the limiting step for Lipofectamin PLUS. These studies indicated the importance of controlling intra-nuclear disposition in the development of an efficient non-viral gene delivery system.
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