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Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Published on: October 20, 2023

Quality assurance program for peripheral blood mononuclear cell cryopreservation.

Adriana Weinberg¹, Raul Louzao, Marisa M Mussi-Pinhata

¹University of Colorado Health Sciences Center, Denver, Colorado, USA. Adriana.Weinberg@uchsc.edu

Clinical and Vaccine Immunology : CVI

|July 27, 2007

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Quality assurance for cryopreserved peripheral blood mononuclear cells (PBMC) improved over time in a pilot program. Training significantly enhanced PBMC viability and recovery for pediatric AIDS clinical trials.

Area of Science:

Immunology
Cell Biology
Clinical Trials

Background:

Cryopreservation of biological samples is crucial for long-term storage and analysis in clinical research.
Ensuring the quality of cryopreserved peripheral blood mononuclear cells (PBMC) is vital for accurate results in studies, particularly in pediatric populations.
The Pediatric AIDS Clinical Trials Group (PACTG) requires high-quality samples for its international research initiatives.

Purpose of the Study:

To assess the quality assurance of cryopreserved PBMC across multiple international sites.
To evaluate the impact of a centralized training program on PBMC cryopreservation quality.
To improve cell viability and recovery rates for samples used in pediatric HIV/AIDS clinical trials.

Main Methods:

Seven Brazilian sites participating in the PACTG cryopreservation quality assurance pilot program.

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Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Published on: October 20, 2023

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

Published on: October 25, 2024

Cryopreservation and shipment of peripheral blood mononuclear cells (PBMC) to a central U.S. laboratory.

Analysis of cell viability and recovery post-cryopreservation.

Implementation of a wet-laboratory training session at the central laboratory.

Main Results:

Significant increases in cell viability and recovery were observed over the duration of the pilot program.
The wet-laboratory training session demonstrated a significant positive impact on the quality of cryopreserved PBMC.
Consistent improvement in PBMC quality indicates successful implementation of quality assurance measures.

Conclusions:

The international cryopreservation quality assurance pilot program successfully improved PBMC quality over time.
Centralized laboratory training is an effective strategy for enhancing cryopreservation techniques in multi-site clinical trials.
Improved PBMC quality supports the reliability of data generated from pediatric AIDS clinical trials.