Related Experiment Video

Updated: Jul 13, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

CEACAM5 and CEACAM6 are major target genes for Smad3-mediated TGF-beta signaling

S-U Han1, T-H Kwak, K H Her

  • 1Laboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Oncogene
|July 27, 2007
PubMed

Insights

Transforming growth factor-beta (TGF-beta) signaling activates carcinoembryonic antigen (CEA) family members, specifically CEACAM5 and CEACAM6, in gastric cancer cells. This pathway is crucial for CEA expression, impacting cell adhesion and differentiation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • The carcinoembryonic antigen (CEA) family comprises diverse glycoproteins involved in cell adhesion and differentiation.
  • Transforming growth factor-beta (TGF-beta) signaling is known to stimulate CEA secretion, but specific targets and mechanisms remain unclear.

Purpose of the Study:

  • To identify specific CEAs regulated by TGF-beta signaling.
  • To elucidate the mechanism underlying TGF-beta-induced CEA expression in gastric cancer.

Main Methods:

  • Analysis of CEA expression in nine human gastric cancer cell lines.
  • Investigating TGF-beta signaling restoration in unresponsive cell lines (SNU638, SNU484).
  • Assessing CEACAM5 and CEACAM6 promoter activity and in vivo expression in Smad3 null mice.

Main Results:

  • TGF-beta-responsive gastric cancer cells exhibited positive CEA expression.
  • Restoring TGF-beta signaling induced CEACAM5 and CEACAM6 expression and promoter activity.
  • CEA expression was reduced in Smad3 null mice, indicating Smad3's role.

Conclusions:

  • CEACAM5 and CEACAM6 are identified as major target genes of Smad3-mediated TGF-beta signaling.
  • TGF-beta signaling plays a significant role in regulating CEA expression in gastric cancer.

More Related Videos

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation09:40

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Published on: June 27, 2017

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System11:06

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System

Published on: July 30, 2018

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...