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Published on: June 13, 2021
Heterologous expression, purification, and characterization of xylose reductase from Candida shehatae
Xiaoxia Wang1, Baishan Fang, Juxiang Luo
1Institute of Industrial Biotechnology, Huaqiao University, 362021, Quanzhou, P.R. China.
Biotechnology Letters
|July 27, 2007
Abstract:
The xylose reductase (XR) gene (xyl1) from Candida shehatae was cloned and expressed in Escherichia coli, and purified as a His(6)-tagged fusion protein. The recombinant XR had K(m) values for NADH than NADPH of 150 microM and 20 microM, respectively. The optimal reaction was at pH 6.5 and 35 degrees C. The enzyme was specific for D-xylese.
