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Updated: Jul 13, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
An electrochemical sensor based on the human estrogen receptor ligand binding domain
Jacob A Hansen1, Vadim V Sumbayev, Kurt V Gothelf
1Department of Chemistry, Aarhus University, 8000 Aarhus C, Denmark.
Abstract:
Three different conformations of the ligand binding domain of the human estrogen receptor (ER-LBD) are observed for the native state when binding an agonist and when binding an antagonist. By conjugating ER-LBD conformation specific peptides to CdS nanoparticles, the three different states can be identified by anodic stripping voltammetry. This electrochemical sensor can detect and distinguish the binding of different ligands to the human estrogen receptor.
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