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Updated: Jul 13, 2026
04:15
A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs
Published on: February 2, 2024
Chicken thrombocytes express the CD51/CD61 integrin
1Institute for Animal Physiology, University of Munich, Veterinärstrasse 13, 80539 Munich, Germany.
Veterinary Immunology and Immunopathology
|July 31, 2007
Summary
Researchers screened 377 anti-human monoclonal antibodies (mab) for chicken cell reactivity. The mab 23C6 specifically identified chicken thrombocytes, marking a significant advancement in avian cell research.
Area of Science:
- Immunology
- Avian Biology
- Cell Biology
Background:
- Monoclonal antibodies (mab) are crucial tools in cell identification.
- Cross-reactivity screening of human-specific mab on avian cells is essential for developing new reagents.
- Chicken leukocyte subsets require specific markers for accurate immunological studies.
Purpose of the Study:
- To screen commercially available anti-human monoclonal antibodies (mab) for cross-reactivity on chicken peripheral blood lymphocytes (PBL).
- To identify specific mab that can reliably identify chicken cell populations.
- To characterize the reactivity and target antigens of promising mab in chickens.
Main Methods:
- Screening of 377 anti-human mab on chicken PBL from two different chicken lines.
- Double immunofluorescence analyses to confirm antigen coexpression.
- Database mining to analyze the genetic loci of chicken CD51 and CD61 genes.
Main Results:
- Only two out of 377 mab showed consistent reactivity with chicken PBL subpopulations.
- Mab HUH73A (anti-CD11a) reacted with all lymphocytes.
- Mab 23C6 (anti-CD51/CD61) specifically reacted with all chicken thrombocytes, which also expressed CD45 and the K1 marker.
Conclusions:
- Mab 23C6 is a highly specific and useful reagent for the identification of chicken thrombocytes.
- The study provides insights into the genetic localization of chicken CD51 and CD61 genes.
- This research facilitates further studies on chicken immune cells and hematopoiesis.
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