[Impact of trichostatin A on gastric carcinoma cell line SGC-7901]
Yun-long Li1, Xiao-ming Zou, Bao-liang Guo
1Department of General Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
Objective:
To investigate the effect of trichostatin A(TSA) on SGC- 7901 cells.
Methods:
Cytotoxicity and cell viability of gastric cancer cell line SGC- 7901 were assayed by MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope. Cell cycle and apoptosis rate were analyzed by flow cytometry. Histone H3 acetylation was detected by Western blot.
Results:
TSA showed apparently cytotoxicity in SGC- 7901 cells. The growth curve showed the growth ratio decreased with the increase of TSA concentration. Apoptosis rate were significantly different between TSA treated group(75 ng/ml for 72 h)and control group (P < 0.05). Morphologic changes of apoptosis including nuclear chromatin condensation and fluorescence strength were observed with fluorescence microscope.TSA treatment (75 ng/ml for 72 h) sensitively induced apoptosis in the cell,which was demonstrated by the migration of many cells to the sub- G1 phase,the reduction of G1- phase cells and the increment of apoptosis rate (29.54%) in flow cytometric analysis. The expression of acetylated histone H3 was increased in TSA group(75 ng/ml) for 48 h compared with control group by Western blot.
Conclusions:
TSA can induce SGC- 7901 cell apoptosis. The expression of acetylated histone H3 may contribute to the apoptosis.
Insights
Trichostatin A (TSA) induces apoptosis in SGC-7901 gastric cancer cells, demonstrated by increased cell death and histone H3 acetylation. This suggests TSA
Area of Science:
- Molecular Biology
- Cell Biology
- Cancer Research
Context:
- Gastric cancer remains a significant global health challenge.
- SGC-7901 is a human gastric adenocarcinoma cell line.
- Trichostatin A (TSA) is a known histone deacetylase inhibitor.
Purpose:
- To investigate the cytotoxic and apoptotic effects of TSA on SGC-7901 cells.
- To analyze the impact of TSA on cell cycle progression and histone acetylation.
- To elucidate the mechanism by which TSA affects gastric cancer cell viability.
Summary:
- TSA demonstrated significant cytotoxicity against SGC-7901 cells in a dose-dependent manner.
- Flow cytometry revealed TSA treatment (75 ng/ml for 72 h) induced apoptosis, evidenced by cell cycle arrest in the sub-G1 phase and a 29.54% apoptosis rate.
- Western blot analysis confirmed increased histone H3 acetylation in TSA-treated cells, suggesting a role in apoptosis induction.
Impact:
- TSA effectively induces apoptosis in SGC-7901 gastric cancer cells.
- Histone acetylation mediated by TSA may be a key factor in its anti-cancer effects.
- These findings provide a basis for exploring TSA as a potential therapeutic agent for gastric cancer.
