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Updated: Jul 13, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
Alexander Egner1, Claudia Geisler, Claas von Middendorff
1Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. aegner@gwdg.de
Abstract:
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.
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