Related Experiment Video
Updated: Apr 26, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Performance of a transcription-mediated-amplification HIV-1 RNA assay in pooled specimens
Nicola M Zetola1, Ann Mintie, Sally Liska
1Division of Infectious Diseases, University of California-San Francisco, San Francisco, CA, United States.
Background:
Very limited data exist on the comparative performance of nucleic acid amplification tests (NATs) for the screening of pooled specimens for acute human immunodeficiency virus (HIV) infection.
Study Design:
In this study, we compared a transcription-mediated-amplification assay (Procleix HIV-1 Discriminatory Assay, [TMA]) with a branched DNA assay (Bayer Versant HIV-1 RNA 3.0 assay, [bDNA]).
Results:
After re-testing 1552 samples that were negative for HIV RNA by bDNA, we found one additional positive sample with the TMA assay.
Conclusion:
Our results suggest that TMA could potentially detect acute HIV infections missed by other technologies.
More Related Videos
08:37Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
07:21Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment
Published on: June 23, 2019