Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Visualization of synapse-glia dynamics].

Hideko Nishida1, Shigeo Okabe

  • 1Department of Cell Biology, School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo ku, Tokyo 113-8519, Japan.

Brain and Nerve = Shinkei Kenkyu No Shinpo
|August 1, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suppression of ATM kinase signaling accelerates cellular senescence.

Stem cell reports·2026
Same author

Localization of Puncta Adherentia Junctions at GABAergic Synapses on Parvalbumin-Positive GABAergic Inhibitory Neurons in the Mouse Hippocampus.

The Journal of comparative neurology·2026
Same author

Intercellular communication in the brain through a dendritic nanotubular network.

Science (New York, N.Y.)·2025
Same author

Intercellular communication in the brain via dendritic nanotubular network.

bioRxiv : the preprint server for biology·2025
Same author

Mice with deficiency in Pcdh15, a gene associated with bipolar disorders, exhibit significantly elevated diurnal amplitudes of locomotion and body temperature.

Translational psychiatry·2024
Same author

Development and Application of Technology for Neural Circuit Visualization - Secondary Publication.

JMA journal·2024

Live imaging reveals dynamic neuron-astrocyte interactions crucial for synaptic plasticity. Astrocytes actively shape neuronal connections, influencing synapse development and function.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Synaptic Plasticity

Background:

  • Neuron-astrocyte interactions are vital for synaptic function, but structural evidence remains limited.
  • Early studies used electron microscopy to show close relationships between synapses and glial processes.
  • Live imaging techniques are essential to observe dynamic synapse-astrocyte interactions.

Purpose of the Study:

  • To investigate the structural dynamics of neuron-astrocyte interactions in live brain tissue.
  • To understand the role of astrocytes in regulating synaptic structure and function.
  • To explore the coordinated morphological dynamics between astrocytic processes and dendritic spines.

Main Methods:

  • Utilized live imaging techniques including transgenic mice (GFAP promoter-GFP), sulforhodamine 101 (SR101), ballistic labeling, and recombinant viruses.

Related Experiment Videos

  • Performed simultaneous observation of filopodia/spines and astrocytes in acute slices and tissue cultures.
  • Monitored astrocyte motility, process extension/retraction, and shape changes in real-time.

    • Main Results:

    • Live astrocytes exhibit significant motility, extending and retracting fine processes.
    • Astrocytic processes and filopodia/spines show coordinated morphological dynamics.
    • Astrocytic contacts were found to regulate filopodial stabilization and spine maturation.

    Conclusions:

    • Astrocytes play an active, dynamic role in shaping synaptic structures.
    • The observed coordinated dynamics suggest functional implications for synapse-astrocyte contacts.
    • Future research should focus on in vivo imaging and correlating astrocytic structural changes with synaptic plasticity.