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High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers
Published on: October 26, 2021
Myrothecium roridum Tri4 encodes a multifunctional oxygenase required for three oxygenation steps
Susan P McCormick1, Nancy J Alexander
1Mycotoxin Research Unit, US Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Avenue, Peoria, IL 61604-3902, USA. susan.mccormick@ars.usda.gov
Abstract:
The biosyntheses of both macrocyclic trichothecenes in Myrothecium roridum and simple trichothecenes in Fusarium species begin with the cyclization of farnesyl pyrophosphate to form the sesquiterpene hydrocarbon trichodiene. A previous study showed that Myrothecium has a cluster of 3 genes that are homologous with Fusarium trichothecene genes: Tri4, a P450 oxygenase; Tri5, the sesquiterpene cyclase; and Tri6, a zinc-finger regulatory gene. Fusarium graminearum Tri4 (FgTri4) and M. roridum MrTri4 (MrTri4) have 66.9% identity. In this study, MrTri4 was expressed in Fusarium verticillioides. Liquid cultures of transformant strains expressing MrTri4 converted exogenous trichodiene to isotrichodiol, indicating that MrTri4 controls 3 oxygenation steps and that the product of MrTRI4 is isotrichodiol.
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