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Updated: Jul 13, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Matching illumination of solid objects
Sylvia C Pont1, Jan J Koenderink
1Helmholtz Institute, Department of Physics and Astronomy, Utrecht University, Utrecht, The Netherlands. s.c.pont@phys.uu.nl
Abstract:
The appearance of objects is determined by their surface reflectance and roughness and by the light field. Conversely, human observers might derive properties of the light field from the appearance of objects. The inverse problem has no unique solution, so perceptual interactions between reflectance, roughness, and lightfield are to be expected. In two separate experiments, we tested whether observers are able to match the illumination of spheres under collimated illumination only (matching of illumination direction) and under more or less diffuse illumination (matching of illumination direction and directedness of the beam). We found that observers are quite able to match collimated illumination directions of two rendered Lambertian spheres. Matching of the collimated beam directions of a Lambertian sphere and that of a real object with arbitrary reflectance and roughness properties resulted in similar results for the azimuthal angle, but in higher variance for the polar angle. Translucent objects and a tennis ball were found to be systematic outliers. If the directedness of the beam was also varied, the direction settings showed larger variance for more diffuse illumination. The directedness settings showed an overall quite large variance and, interestingly, interacted with the polar angle settings. We discuss possible photometrical mechanisms behind these effects.
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