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Published on: November 28, 2019
[Effect of lipopolysaccharide on antitumor activity of mouse kupffer cells in vitro]
Zhi-Wei Zhou1, De-Sen Wan, Xiao-Dong Sun
1State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, PR China. zhouzhiw@pub.guangzhou.gd.cn
Background & Objective:
Kupffer cells (KCs) in the liver constitute the largest group of macrophages with strong immune functions. This study was to investigate the effect of lipopolysaccharide (LPS) on antitumor activity of mouse KCs in vitro.
Methods:
KCs were isolated from the mouse liver by enzyme digestion, and co-cultured with mouse colon cancer cell line CT26 at different effector/target (KCs:CT26) ratios. The inhibitory effect of KCs on the proliferation of CT26 cells at different effector/target ratios, or at a ratio of 10:1 with treatment of LPS was measured by WST-8 colorimetric assay.
Results:
The inhibition rate of CT26 cell proliferation was increased along with ascending effector/target ratios: the inhibition rates were 7.86%, 13.37%, 15.51%, 18.86%, 18.79%, 19.24%, and 19.98% when the effector/target ratios were 1:1, 2:1, 5:1, 10:1, 20:1, 50:1, and 100:1, respectively. At an effector/target ratio of 10:1, the inhibitory effect of KCs on CT26 cell proliferation was enhanced by LPS: the inhibition rates were 16.86%, 21.1%, 25.64%, 28.38%, 26.78%, 33.38%, and 38.7% when treated with 0.001, 0.01, 0.1, 1, 10, 100, and 1,000 microg/ml LPS, respectively.
Conclusion:
LPS may activate KCs in vitro, and enhance its antitumor activity as well.
