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[Content determination of bergenin in Ardisia pusilla by HPLC]
1Medicine College of South-west Jiao-tong University, Emei 614202, China.
Objective:
To determine the content of bergenin in Ardisia pusilla.
Methods:
High Performance Liquid Chromatography was performed on Hyersil C18ODS. The chromatorgraphic conditions were as follows: methanol-water (20:80) as mobile phase, flow rate being 0.600 ml/min and detecting wavelength at 275 nm. The column temperature was 25 degrees C.
Results:
A good linearity of bergenin was shown in range of 0. 098-0. 591 microg (r = 0.9998), the average recovery is 102.0%, RSD = 1.4% (n=6). The content of bergenin in Ardisia pusilla is 59% at least.
Conclusion:
The content of bergenin in Ardisia pusilla and A. japonica is identical.
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