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[Fingerprint analysis of Luotong capsule by HPLC]
Xiao-li Zhao1, Liu-qing Di, Hao Wu
1Institue of TCM, Nanjing University of Traitional Chinese Medicine, Nanjing 210029, China. xlee_zhao@163.com
Objective:
To develop the chromatographic fingerprints of Luotong capsule by HPLC.
Methods:
The separation was performed on YMC-PacK ODS (4.6 mm x 250 mm, 5 microm) column with a mobile phase consisting of acetonitrile -0.5% phosphoric acid as gradient elution at the flow rate of 1.0 ml/min. The detective wavelength was 276 nm. The column temperature was 30 degrees C.
Results:
A standard HPLC fingerprint procedure was developed for Luotong capsule with 18 common peaks. The similarity of 10 batches of Luotong capsule was not lower than 0.978.
Conclusion:
The mehtod is accurate, repeatable and useful for the quality control of Luotong capsule.
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