Incoherent light transport in an anisotropic random medium: a probe of human erythrocyte aggregation and deformation

This paper investigates the flow induced disaggregation, deformation and orientation of several modified human red blood cells suspended in concentrated, physiological like conditions (volume fraction in erythrocytes of 0.4). The aim is to determine simultaneously, and under flow, the aggregate sizes as well as the deformation and orientation of the cells. The measurement method uses steady, incoherent, unpolarized light transport while the sample is sheared in a flow cell controlled by a rheometer. Several blood samples were prepared to alter the erythrocyte's aggregating, deformability and shape properties. The measurements using these samples show a clear relationship between the intrinsic properties of the cells and the evolution of aggregate sizes, average cell orientation and anisotropy as a function of the applied shear, which may lead to clinical applications. In other words, the careful analysis of the incoherent light transport in concentrated media provides quantitative insight into their microscopic details. In particular, the topological properties (average anisotropy and orientation) and size of the suspended objects can be determined.