[Determination of flavonoid glycosides in Pyrola decorate by reversed-phase high performance liquid chromatography]

A reversed-phase high performance liquid chromatographic method was developed for the simultaneous determination of five flavonoid glycoside components, namely hyperin, isoquercitrin, 2"-O-galloylhyperin, quercetin 3-O-alpha-L-arabinofuranoside and quercitrin in Pyrola decorate. After being immersed in 70% (v/v) ethyl alcohol for 6 h, samples were ultrasonically extracted for 30 min. The separation was carried out on a Zorbax Extend-C18 column (250 mm x 4.6 mm, 5 microm) using acetonitrile-water (14 : 86, v/v) as mobile phase at a flow rate of 1.0 mL/min. The absorbance was monitored at 350 nm and the column temperature was 35 degrees C. All analytes showed good linearity within the test ranges and the recoveries were 96.3% - 104.2%. Satisfactory precisions were obtained with relative standard deviations less than 5%. The method was simple, accurate and reproducible in the application to the determination of 20 commercial samples of Pyrola decorata collected from different regions of China. It can be used for the quality control of both plant materials and preparations of Pyrola decorate.