Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Open, reconfigurable cytometric acquisition system: ORCAS.

Mark A Naivar1, Jimmie D Parson, Mark E Wilder

  • 1Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA. mnaivar@lanl.gov

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|August 8, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tailoring the Relational Playbook for Cardiology Teams to the Rural Hospital Medicine Setting: A Multi-Method Pre-Implementation Evaluation.

The Journal of rural health : official journal of the American Rural Health Association and the National Rural Health Care Association·2026
Same author

Acoustofluidic trapping of microparticles to axially centered wires in cylindrical microcapillaries.

Lab on a chip·2026
Same author

Transgenic hookworm secretes anti-tetrodotoxin human single chain antibody.

Nature communications·2026
Same author

Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer's Disease Cerebrocortical Organoids.

bioRxiv : the preprint server for biology·2026
Same author

Analysis of National Institutes of Health funding for back pain research in the United States.

North American Spine Society journal·2026
Same author

Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles.

Nature protocols·2026
Same journal

The 1st Mediterranean Meeting on Flow Cytometry: Forging New Collaborations Across the Mediterranean and Beyond.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Publication Guidelines for Optimized Multiparameter Immunolabeling Panels (OMIPs).

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

A Modular High-Parameter Flow Cytometry Framework: Pre-Analytical Optimization and Validation for Clinical Research.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Quantitative Detection of Entotic Cell-In-Cell Structures Using Deformable Segmentation and Deep Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
See all related articles

A novel digital data acquisition system uses digital signal processing (DSP) to enhance flow cytometry. This flexible system captures and processes event data with high sensitivity and linearity, enabling advanced analysis.

Area of Science:

  • Biotechnology
  • Data Acquisition Systems
  • Signal Processing

Background:

  • Traditional flow cytometry systems have limitations in data capture and processing flexibility.
  • Novel applications require advanced data acquisition capabilities beyond commercial systems.

Purpose of the Study:

  • To develop a flexible, digital signal processing (DSP)-based digital data acquisition system for advanced flow cytometry.
  • To enable the capture and processing of diverse data types, including correlated pulse shapes and streaming data.

Main Methods:

  • Custom data capture boards with analog-to-digital converters (ADCs) and field-programmable gate arrays (FPGAs) were developed.
  • A commercial DSP board processed captured data, transmitting results to a host computer via IEEE 1394 bus.

Related Experiment Videos

  • The system supported Linux, Mac OS X, and Windows operating systems for data acquisition, display, analysis, and storage.

    • Main Results:

    • The system demonstrated excellent sensitivity and linearity in extracting pulse features over a wide dynamic range.
    • It successfully collected list mode data, correlated pulse shapes, and streaming data from various detector types.
    • List mode data were saved in FCS 3.0 format, while waveform data were stored in custom formats for postprocessing.

    Conclusions:

    • The developed open, reconfigurable cytometric acquisition system is compact, scalable, flexible, and modular.
    • Programmable feature extraction algorithms offer significant potential for new and existing flow cytometry applications.
    • The availability of commercial data capture boards will facilitate the broader adoption of similar systems.