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Adsorption and expression of penicillin G acylase immobilized onto methacrylate polymers generated with varying pore
A Kotha1, L Selvaraj, C R Rajan
1Chemical Engineering Division, National Chemical Laboratory, Pune, India.
Applied Biochemistry and Biotechnology
|September 1, 1991
Abstract:
Adsorption and expression of penicillin G acylase was studied on macroporous methacrylate polymer beads of differing pore volume, generated with kerosene. The absorption and expression of the penicillin G acylase was dependent on pore volume. Maximum expression of 57% of adsorbed enzyme was obtained on beads synthesized with 40 mL of kerosene, indicating minimum pore-diffusion limitations.