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Updated: Jul 13, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Chemiluminescence of luminol catalyzed by silver nanoparticles
1Department of Bio-Nano Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Ministry of Education, Research Institute of Micro & Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, PR China 200030.
Abstract:
Silver nanoparticles (AgNPs) are synthesized by chemical reduction method and characterized by UV-vis spectra, transmission electron microscopy, and high performance particle sizer. We have found that AgNPs could enhance the chemiluminescence (CL) intensity of luminol-H(2)O(2) system. In this reaction, luminol intermediate is generated under alkaline condition on the surface of AgNPs in luminol-H(2)O(2) system and enhances CL intensity. To validate the reaction mechanism, AgNPs are bound with thioglycolic acid (Ag-HSCH(2)COOH) and then joined to BSA protein (Ag-BSA). We investigate the CL intensity in the presence of Ag-HSCH(2)COOH or Ag-BSA comparing with that in the presence of AgNPs and conclude the catalytic reaction take place on the surface of AgNPs.
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