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Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Size and Structure of Viral Genomes

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Related Experiment Video

Updated: Jul 13, 2026

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
06:25

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Published on: July 10, 2019

Are all lyssavirus genes equal for phylogenetic analyses?

Xianfu Wu1, Richard Franka, Andres Velasco-Villa

  • 1Rabies Program/PRB, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA. XAW6@cdc.gov

Virus Research
|August 8, 2007
PubMed
Summary

This study sequenced the ERA rabies virus genome, revealing gene similarities. Lyssavirus genes likely hold comparable value for phylogenetic analysis, aiding rabies virus research.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Lyssavirus genus includes rabies virus, a significant public health concern.
  • Phylogenetic analysis of lyssavirus genes is crucial for understanding viral evolution and transmission.

Purpose of the Study:

  • To sequence the full genome of the ERA rabies virus strain.
  • To evaluate the phylogenetic utility of individual lyssavirus genes using comprehensive datasets.

Main Methods:

  • Full genome sequencing of the ERA rabies virus.
  • Virus recovery and confirmation via reverse genetics.
  • Nucleotide diversity, protein co-variation, and structural/functional constraint analyses.
  • Phylogenetic tree reconstruction.

Main Results:

  • The complete ERA genome sequence (11,931 nt) was determined, detailing gene lengths and intergenic regions.
  • A leaky termination signal (TGpoly(A(8))) was identified in the ERA strain's glycoprotein gene.
  • Comparative analysis revealed differences in polyadenylation signals between ERA and SAD-B19 strains.

Conclusions:

  • Individual lyssavirus genes possess similar phylogenetic value.
  • Comprehensive genomic and genetic analyses support robust phylogenetic inferences for lyssaviruses.