[Determination of catechelamines, serotonin, and 5-hydroxyindoleacetic acid in a blood]

The specific features of chromatographic electrochemical detector determination of plasma catecholamines, serotonin, and 5-hydroxyindoleacetic acid (5-HIAA) are considered. Platelet-poor plasma catecholamines were concentrated on alumina. The concentrate was injected into a chromatograph. Serotonin and 5-HIAA were measured in platelet-poor plasma and thrombocytic clot. The data available in the literature and their authors' own findings of the sample preparation parameters that could minimize the artificially increased platelet-poor plasma concentrations of serotonin via thrombolysis, as well its loss on protein sedimentation.