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Updated: Jul 13, 2026
07:47
Experimental Human Pneumococcal Carriage
Published on: February 15, 2013
Prevotella pleuritidis sp. nov., isolated from pleural fluid
Mitsuo Sakamoto1, Kiyofumi Ohkusu2, Takayuki Masaki3
1Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Summary
A novel bacterium, Prevotella pleuritidis, was identified from pleural fluid. This anaerobic Gram-negative rod represents a new species within the Prevotella genus, distinct from its closest relative Prevotella enoeca.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Suppurative pleuritis can be caused by various bacteria.
- The genus Prevotella comprises anaerobic Gram-negative bacteria, often associated with infections.
Purpose of the Study:
- To characterize a novel bacterial strain isolated from a patient with suppurative pleuritis.
- To determine the phylogenetic and taxonomic position of the isolate.
Main Methods:
- Phenotypic and biochemical characterization.
- Cellular fatty acid profiling.
- 16S rRNA gene sequence analysis for phylogenetic placement.
Main Results:
- The isolate, strain GTC 3021(T), is an obligately anaerobic, Gram-negative rod.
- 16S rRNA gene sequence analysis placed the isolate within the Prevotella genus, with 92% similarity to Prevotella enoeca.
- Significant differences in cellular fatty acid composition distinguished the isolate from P. enoeca.
- A new species, Prevotella pleuritidis sp. nov., is proposed with strain GTC 3021(T) as the type strain.
Conclusions:
- Strain GTC 3021(T) represents a novel species, Prevotella pleuritidis, based on phenotypic, biochemical, and phylogenetic data.
- This finding expands the known diversity within the Prevotella genus.
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