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Updated: Jul 13, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
3H-serotonin as a marker of oscillatory insulin secretion in clonal beta-cells (INS-1)
Jude T Deeney1, Robert Bränström, Barbara E Corkey
1The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, S-171 76, Stockholm, Sweden. jdeeney@bu.edu
Abstract:
Serotonin release from preloaded pancreatic beta-cells has been used as a marker for insulin release in studying exocytosis from single cells using the amperometric technique. We found that single pancreatic beta-cells exhibited oscillations in exocytosis with a period of 1-1.5 min as measured amperometrically by serotonin release. We also show that 3H-serotonin can be used to monitor exocytosis from intact and streptolysin-O permeabilized clonal insulin-secreting cells preloaded with labeled serotonin and that serotonin release correlated with insulin secretion in the same cells. The use of 3H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells.
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